bicarbonate-buffered tissue culture medium 199 (Thermo Fisher)
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bicarbonate-buffered tissue culture medium 199
Bicarbonate Buffered Tissue Culture Medium 199, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bicarbonate-buffered+tissue+culture+medium+199/pm39742720-72-11-17
Average 90 stars, based on 1 article reviews
Bicarbonate Buffered Tissue Culture Medium 199, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bicarbonate-buffered+tissue+culture+medium+199/pm39742720-72-11-17
Average 90 stars, based on 1 article reviews
bicarbonate-buffered tissue culture medium 199 - by Bioz Stars,
2026-09
90/100 stars
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other:Article Title: Effect of mSOF and G1.1/G2.2 media on the developmental competence of SCNT-derived bovine embryos. Article Snippet: The objective of this study was to compare the effect of two culture media: modified synthetic oviductal fluid (mSOF) and G1.2 ⁄G2.2, on the developmental competence of bovine somatic cell–cloned embryos.. Cloned embryos were produced by transferring adult skin fibroblasts into enucleated MII oocytes.. After activation, the reconstructed embryos were randomly allotted to either mSOF or G1.2 ⁄G2.2 for culture (the embryos were transferred from G1.2 to G2.2 on days 3 of culture). Article Title: Zinc-finger nickase-mediated insertion of the lysostaphin gene into the beta-casein locus in cloned cows Article Snippet: Only COCs with compact cumulus and evenly granulated cytoplasm were collected and incubated in bicarbonate-buffered tissue culture medium 199 (TCM-199; Article Title: Transgenic cattle produced by nuclear transfer of fetal fibroblasts carrying Ipr1 gene at a specific locus. Article Snippet: This study aimed to assess the effects of the intracellular pathogen resistance 1 (Ipr1) transgene on preventing infection of Mycobacterium bovis in cattle.. A specific expression vector for the Ipr1 gene was constructed and inserted in the genome between surfactant protein A and methionine adenosyltransferase I of bovine fetal fibroblasts.. After SCNT, cleavage (86.9% vs. 87.4%, P > 0.05) and blastocyst developmental rates (34.6% vs. 33.5%, P > 0.05) were similar between transgenic and nontransgenic bovine fetal fibroblasts. Article Title: Effect of extracellular vesicles derived from oviductal and uterine fluid on the development of porcine preimplantation embryos. Article Snippet: To improve the efficiency of in-vitro-produced (IVP) porcine embryos, we focused on the events that usually occur during in-vivo embryonic transit from the oviduct to the uterus.. Extracellular vesicles (EVs) are released by different mammalian cells and are imperative for intercellular communication and reflect the cell’s physiological state.. Based on these characteristics, EVs were isolated from oviductal and uterine fluid to imitate the in vivo environment and improve the efficiency of IVP embryos. Article Title: Development of bovine-ovine interspecies cloned embryos and mitochondria segregation in blastomeres during preimplantation. Article Snippet: The objective of the study was to investigate interspecies somatic cell nuclear transfer (iSCNT) embryonic potential and mitochondrial DNA (mtDNA) segregation during preimplantation development.. We generated bovine–ovine reconstructed embryos via iSCNT using bovine oocytes as recipient cytoplasm and ovine fetal fibroblast as donor cells.. Chromosome composition, the total cell number of blastocyst and embryonic morphology were analyzed. |